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© Thibault Andrieux


FACTS & HIGHLIGHTS OF THE PROJECT (2015-2019):
We have progressed substantially on the development of a cost-effective mass-rearing protocol for SWD and we have developed an “oviposition” system” which is very promising. We have finalized all the irradiation assays necessary to develop SIT for SWD. Quality control procedures were developed. Two promising candidate strains of Wolbachia to develop biocontrol with IIT in SWD were found. These Wolbachia strains induce strong cytoplasmic incompatibility that was not rescued by the native strain wSuz and transinfected males showed the same competitiveness as the “natural” males. Collectively these results allowed to develop a promising combined SIT/IIT protocol using these Wolbachia strains and a low irradiation dose. We evaluated the impact of this procedure on crucial quality control parameters and showed that this low irradiation dose did not negatively affect the fly populations. We have also gained significant knowledge on the cold tolerance of our lab-domesticated SWD lines and found useful and practical methods for conditioning flies before release, for cold storage (short and long term) and for cold transportation. Finally, we investigated the genetic changes occurring during domestication process and showed that the population genetic structure was changing as the domestication was progressing in the laboratory.



International conferences and Workshops :

Colinet H. Exploring the benefits of fluctuating thermal regimes on cold tolerance of Drosophila flies by combined metabolomic and lipidomic approach. Second FAO/IAEA Research Coordinated Meeting on CRP D41025: Dormancy Management to Enable Mass-rearing and Increase Efficacy of Sterile Insects and Natural Enemies. Stellenbosch, South Africa, 18-22 March 2016.

Cattel J., Andrieux T., Martinez J., Jiggins F., Charlat S., Vavre F., Gibert P. & Mouton L. Identification of a candidate Wolbachia strain for developing the Incompatible Insect Technique to control Drosophila suzukii populations Conference : 9th International Wolbachia conference. Queensland (Australia). Juin 2016.

Cattel J., Andrieux T., Martinez J., Jiggins F., Charlat S., Vavre F., Gibert P., Mouton L. Incompatible Insect Technique: a promising approach to control Drosophila suzukii populations. workshop: “Sustainability of agro-ecological pest management: tackling ecological, evolution and sociological challenges with fruit-fly research”. Montpellier (France). September 2016.

Colinet, H., Enriquez, T. Assessment of basal thermal tolerance of Drosophila suzukii and optimizing storage conditions under constant and/or fluctuating cold conditions. Third FAO/IAEA research coordination meeting on Dormancy Management to Enable Mass-rearing and Increase Efficacy of Sterile Insects and Natural Enemies. 29 May to 2 June 2017, Vienna, Austria.

Enriquez T., Renault, D., Charrier, M., Colinet, H. Cold acclimation favors metabolic stability in Drosophila suzukii. 7th International Symposium of Environmental Physiology of Ectotherms and Plants (ISEPEP7). Tartu (Estonia) 31 july-04 August 2017.

Nikolouli, K., Sassu, F., Enriquez, T., Gibert, P., Vavre, F., Mouton, T., Renault, D., Stauffer, C., Caceres, C., Bourtzis, K., Cardoso-Pereira, R., Colinet, H. Managing cold tolerance and quality of mass-produced Drosophila suzukii flies to facilitate the application of biocontrol through incompatible and sterile insect techniques. Third FAO–IAEA International Conference on Area-wide Management of Insect Pests, Vienna Vienna May 22-26 – 2017

Sassu F., Caceres C., Pereira R., Stuffer C. Development of the Sterile Insect Technique to Manage Confined Populations of Drosophila suzukii. Third FAO–IAEA International Conference on Area-wide Management of Insect Pests, Vienna Vienna May 22-26, 2017

Nikolouli K, Mouton L, Stauffer C, Bourtzis K. Could insect genetics and symbiosis contribute to the development of SIT-based applications against Drosophila suzukii? 'FIRST FAO/IAEA RESEARCH COORDINATION MEETING ON Integration of the SIT with Biocontrol for Greenhouse Insect Pest Management, Vienna, 3 – 7 July 2017.

Sassu F., Caceres C., Pereira R., Stuffer C. Progress on the development of SIT for SWD at IPCL. 'FIRST FAO/IAEA RESEARCH COORDINATION MEETING ON Integration of the SIT with Biocontrol for Greenhouse Insect Pest Management, Vienna, 3 – 7 July 2017.

Grumiaux, C., Colinet, H., Andersen, M., Overgaard, J. Storing insects for biological control: Fluctuating Thermal Regimes ensures maintenance of physiological homeostasis and reproductive capacity in Drosophila suzukii. SEB Annual Meeting, Florence, Italie, 03-06 juillet 2018.

De Ro M., Enriquez T., Bonte J., Berkvens N., Casteels H., Van Kerckvoorde V., Beliën T., De Clercq P. and Colinet H. Effect of starvation on the cold tolerance of Drosophila suzukii (Diptera: Drosophilidae) adults. 70th International Symposium on Crop Protection, Ghent, Belgium 22 May 2018.

Katerina Nikolouli, Laurence Mouton, Christian Stauffer, Kostas Bourtzis. Characterization of Wolbachia-infected Drosophila suzukii lines for the development of a combined SIT and IIT approach. Sfecologie 2018 - International Conference on Ecological Sciences, Rennes. 23-25 October 2018.

Colinet, H., Enriquez, T., Renault,D., Charrier, M. Eco-physiological aspects of cold tolerance plasticity in Drosophila suzukii. Sfecologie 2018 - International Conference on Ecological Sciences, Rennes. 23-25 October 2018.

Enriquez, T. & Colinet, H. Cold acclimation triggers major transcriptional changes in Drosophila suzukii. Sfecologie 2018 - International Conference on Ecological Sciences, Rennes. 23-25 October 2018.

Colinet, H. Cold tolerance, storage, acclimation and transportation of Drosophila suzukii. Fourth FAO/IAEA research coordination meeting on Dormancy Management to Enable Mass-rearing and Increase Efficacy of Sterile Insects and Natural Enemies. Thessaloniki, Greece. 2-6 June 2019.

National conferences and Workshops :

Mouton, L. La technique de l’insecte incompatible: une approche prometteuse pour lutter contre Drosophila suzukii. Journées Drosophila suzukii. Centre CTIFL de Balandran (France). December 2016.

J. Cattel, F. Vavre, S. Charlat, T. Andrieux, P. Gibert, L. Mouton ''Wolbachia, un agent de lutte biologique efficace pour lutter contre Drosophila suzukii ? " REID 2016, Poitiers (France), 29-31-Mar-2016

Enriquez T & Colinet H. Description des limites thermiques basales de Drosophila suzukii. GDR PlasPhen. 19-20 sept 2016. Paris (France).

Schuler H, Stauffer C. Wolbachia: Ein bakterieller Endosymbiont zur Bekämpfung von Schadinsekten. Arbeitsgemeinschaft für Lebensmittel-, Veterinär- und Agrarwesen (ALVA 2017), Wesenufer – Innviertel (Austria), 22.-23. May.2017.

Colinet H. Projet Suzukill (SIT et IIT) : « Recherche et innovations pour la performance et la durabilité des écosystèmes productifs ». Groupe de travail national « Drosophila suzukii ». CTIFL Balandran. 26 septembre 2017.

Colinet, H., Enriquez, T., Kustre, A. Normes de réaction thermiques chez Drosophila suzukii. 4ème réunion du GDR Plasticité Phéntoypique. Lyon, France, 08-09 octobre 2018.

Mouton L. Combiner la technique de l’insecte stérile à la technique de l’insecte incompatible pour contrôler les populations de Drosophila suzukii. Saint Laurent d’Agny. Octobre 2018.

Publications :

Nikolouli, K., Colinet, H., Renault, D., Enriquez, T., Mouton, T., Gibert, P., Vavre, F., Sassu, F., Caceres, C., Stauffer, C., Cardoso-Pereira, R. and Bourtzis, K. 2018. Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii. Journal of Pest Science. 91 : 489–503

Enriquez T & Colinet H. 2017. Basal tolerance to heat and cold exposure of the spotted wing drosophila, Drosophila suzukii. Peer J. 5:e3112.

Cattel J., Nikolouli K., Andrieux T., Martinez J., Jiggins F., Charlat S., Vavre F., Gibert P., Mouton L. (2018). Back and forth Wolbachia transfers reveal efficient strains to control Drosophila suzukii populations. Journal of Applied Ecology, 55 : 2408-2418

Enriquez, T., Ruel, D., Charrier, M., Colinet, H. Effects of fluctuating thermal regimes on cold survival and life history traits of the spotted wing Drosophila (Drosophila suzukii, Matsumara). Insect Science. DOI 10.1111/1744-7917.12649

Enriquez, T., Renault, D., Charrier, M., Colinet, H. 2018. Cold acclimation favors metabolic stability in Drosophila suzukii. Frontiers in Physiology. 9:1506.

Oliva, C., Mouton, L., Colinet, H., Debelle, A., Gibert, P., Fellous, S. 2019. Principes, mise en œuvre et perspectives de la technique de l’insecte stérile. Dans Biocontrole : éléments pour une protection agroécologique des cultures. (Eds : Fauvergue X et al). QUAE Editions, In press.

Enriquez, T. & Colinet, H. 2019. Cold acclimation triggers lipidomic and metabolic adjustments in the spotted wing drosophila Drosophila suzukii (Matsumara). American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 316: R751–R763.

Enriquez T & Colinet. H. 2019. Cold acclimation triggers major transcriptional changes in Drosophila suzukii. BMC Genomics. 20:413

Sassu, F.; Nikolouli, K.; Caravantes, S.; Taret, G.; Pereira, R.; Vreysen, M.J.B.; Stauffer, C.; Caceres, C. Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems. Insects 2019, 10, 448.

Grumiaux, C., Andersen, M.K., Colinet, H., Overgaard, J. 2019. Fluctuating thermal regime preserves physiological homeostasis and reproductive capacity in Drosophila suzukii. Journal of Insect Physiology. 113: 33-41

Sassu F, Nikolouli K, Pereira R, Vreysen MJB, Stauffer C, Caceres C (2019) Irradiation dose response under hypoxia for the application of the sterile insect technique in Drosophila suzukii. PLoS ONE 14(12): e0226582. https://doi.org/10.1371/journal.pone.0226582

Nikolouli, K., Sassu, F., Mouton, L. et al. Combining sterile and incompatible insect techniques for the population suppression of Drosophila suzukii. J Pest Sci 93, 647-661 (2020)

SUBMITTED :

De Ro M., Enriquez T., Bonte J., Ebrahimi N., Casteels H., De Clercq P. and Colinet H. Effect of starvation on the cold tolerance of adult Drosophila suzukii (Diptera: Drosophilidae).

Enriquez T., Sassù F., Cáceres C., Colinet H. Hypoxia combined with chilling maintains quality of irradiated Drosophila flies: a simulated shipment experiment.

© ECOBIO            Last modification on 13 March 2020